University of Pennsylvania researchers have found a way to avoid the systemic inflammation common in dialysis patients by temporarily suppressing complement during dialysis treatments. The study appears online in Immunobiology ahead of print.
According to the researchers, inflammation can be triggered when the complement cascade, part of the body's innate immune system, is inadvertently activated by modern polymer-based dialysis blood filters.
Over the past several years, lead author John Lambris, PhD, the Dr. Ralph and Sallie Weaver Professor of Research Medicine, Perelman School of Medicine at the University of Pennsylvania, and his colleagues have developed small molecule versions of the drug compstatin, which inhibits a component of the complement immune response called C3. Lambris explains that this next-generation compound, called Cp40, "is a small peptide similar to cyclosporine in many aspects, however it uses a different mechanism of action."
Previous studies by Lambris and his team, in which modern polymer-based hemodialysis filters were perfused with human blood, showed significant complement activation and an increase in inflammatory biomarkers. This response could be suppressed using compstatin, suggesting that it might be used in dialysis to decrease the inflammatory response side effect.
The new study took place in non-human primates to validate Cp40's complement-inhibiting properties in whole animals. Even after undergoing a single session of dialysis using a pediatric hemodialysis filter with high biocompatibility, healthy animals showed strong complement activation with 5% of their C3 being converted to a form that can trigger inflammation and stimulate the immune system.
"This is a huge amount of activation because hemodialysis patients go every two or three days, three times a week, for treatment,” says Lambris. Such repetitive complement activation may create a cytokine boost in humans that could fuel the chronic inflammatory response in renal disease patients. When a single dose of the Cp40 compound was administered, the animals displayed a complete suppression of complement, as indicated by C3 activation levels.
A major advantage of this new approach is that it's short-term, according to the researchers. "The treatment is only for the time of hemodialysis," Lambris explains. "It's not a lasting inhibition. You start hemodialysis, you give the compound, and you inhibit during hemodialysis. After the procedure, the complement system quickly regains its full activity." This avoids potential concerns about adverse effects caused by long-term complement suppression. Cp40 can also be manufactured at relatively low cost, easing the already-high financial burden of maintenance dialysis for ESRD patients.
"Technical challenges make it almost impossible to perform these studies in mice or other small animals in a clinically relevant context, so you have to go to another animal model," said study co-author Daniel Ricklin, PhD, research assistant professor of Pathology and Laboratory Medicine. "I think this new monkey model that we established is a very important step in evaluating disease mechanisms and novel routes of inhibition for this indication."
The chances for clinical translation of this novel therapeutic approach are promising since AMY-101, a drug that is based on Cp40, is currently under clinical development, said Lambris.
Lambris and Ricklin are the inventors of patents and/or patent applications that describe the use of complement inhibitors for therapeutic purposes. Lambris is the founder of Amyndas Pharmaceuticals, which is developing complement inhibitors for clinical applications.